杨浩，博士，研究员，博士生导师，四川大学华西医院国家卫健委移植工程与移植免疫重点实验室PI、科研党委委员，党支部书记，四川省卫健委学术技术带头人后备人选。本科毕业于四川大学生命科学学院生物学基地专业；2010年于四川大学华西医院国家卫健委移植工程与移植免疫重点实验室获得临床医学博士学位；毕业后继续在医院从事博士后研究，2013年出站留院工作。先后获得四川省优秀博士论文、四川大学优秀博士后，四川大学华西医院优秀科研先进个人、第七届华西青年榜样、iGEM国际遗传工程机器大赛金奖指导老师。工作期间先后至斯坦福大学、加州大学河滨分校、赫尔辛基大学和利物浦大学进行段访交流。
研究方向主要聚焦于引起实体器官功能衰竭的肿瘤、感染、衰老等重大临床问题，发展蛋白质组学相关分析技术，建立疾病特征图谱，发现和筛选关键靶点。同时进行多价态模块化蛋白质药物的设计与开发，为相关疾病的靶向诊断与干预提供新途径。负责设计和建立了华西医院蛋白质组学平台并指导平台的运行工作。
担任全球抗击新冠质谱联盟成员（中国大陆6人），中国研究型医院学会临床数据与样本资源库专业委员会委员，四川省质谱专委会理事，四川省抗衰老科学研究促进会理事、青委会主任委员。并任Science Bulletin、Exploration、Theranostics、eBioMedicine、Advanced Materials、ACS Nano、Analytical Chemistry等国内外高水平期刊的同行评阅人。
致力于发现并建立重大疾病的生物标志物图谱及其辅助分类系统，并进行模块化、多价态创新蛋白质药物的研发，主要应用于肿瘤、感染性疾病和器官移植等领域。针对单体蛋白质药物效价不足，建立了系列高价态蛋白质药物的开发策略，突破了当前主要依赖抗体骨架的局限性，创新性地解决了高价态蛋白质药物在结构设计和制备上的难点。先后实现了高价态死亡受体激动剂、人工抗体和ADCs/PDCs等药物的设计与定制。负责建立了华西医院蛋白质组学平台和技术团队，开发和建立了系列大规模临床蛋白质学分析技术、数据质控及分析和可视化方法，成为西部地区的高端技术枢纽，支撑了大量高水平研究和人才培养。基于高分辨质谱技术，建立了针对多价蛋白质药物的表征评价平台，为相关新药创制提供了重要支撑。
先后主持科技部重点研发计划子课题、国家自然科学基金面上项目和省科技厅国际合作项目等10余项研究。相关研究工作近年来以通讯作者（含共同）在Angewandte Chemie International Edition、PNAS、Nucleic Acids Research、ACS Nano、Journal of Controlled Release、Theranostics、Analytical Chemistry等高水平期刊发表论文40余篇，包括4篇封面论文。相关研究成果被Cell、Science Immunology、STTT、Nat Metabolism、Immunity等期刊广泛引用和正面评价。获授权中国发明专利11项，美国发明专利2项，1项专利成果已进入临床IIT研究。通过合作研究，支撑了团队相关成果在Nature Biomedical Engineering、Nature Structural & Molecular Biology、STTT、Mol Cell等期刊发表。
近年来重要代表论文：
1. Chen Y, Huang Y, Wang S, Wang X, Lu H, Chen J, Li J, Chen Z, Li Z, She T, Jin Y, Gao Y, Zhang J, Wang L, Zeng W, Zhu H, Tao Z*, Lu X*, Yang H*. A Self-Assembled Protein Platform for Plug-and-Play Customization of Multivalent Artificial Antibodies and Antibody-Drug Conjugates. Angew Chem Int Ed Engl. 2025;64(40):e202506426. Inside Cover Story.
2. Zhao J, Deng Y, Xu Z, Xu C, Zhao C, Li Z, Sun H, Tian X, Song Y, Cimadevila M, Wang H, Liu Y, Zhang X, Chen Y, Sun S, Yong X, Su L, He Y, Zhong Y, Yang H, Pin JP, Yan W, Shao Z, Liu J. Structural characterization of five functional states of metabotropic glutamate receptor 8. Mol Cell. 2025;85(18):3460-3473 e3466.
3. Zeng J, Ma M, Tao Z, Rao Z, Wu C, Yin S, Jiang X, Chen G, Wang Z, Huang D, Zhu M, Liu L, Huo W, Yang H, Guo H, Chen G, Li F, Zheng C, Huang D, Rahfeld P, Kizhakkedathu JN, Withers SG, Lu X, Zhang K, Lin T, Song T. Enzyme-converted O kidneys allow ABO-incompatible transplantation without hyperacute rejection in a human decedent model. Nat Biomed Eng. 2025.
4. Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W. Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS. Nat Struct Mol Biol. 2025;32(5):896-904.
5. Yang Y, Wang S, Chen Y, Wang X, Jiang W, Jin Y, Zeng W, Wu D*, Shen B*, Yang H*. Ontolomics-P: Advancing Proteomics Data Interpretation through GPT-4o Reannotated Topic Ontology and Data-Driven Analysis. Anal Chem. 2025;97(19):10299-10308.
6. Xiao X, Hu M, Gao L, Yuan H, Chong B, Liu Y, Zhang R, Gong Y, Du D, Zhang Y, Yang H, Liu X, Zhang Y, Zhang H, Xu H, Zhao Y, Meng W, Xie D, Lei P, Qi S, Peng Y, Tan T, Yu Y, Hu H, Dong B, Dai L. Low-input redoxomics facilitates global identification of metabolic regulators of oxidative stress in the gut. Signal Transduct Target Ther. 2025;10(1):8.
7. Tao Z, Li Y, Huang Y, Hu L, Wang S, Wan L, She T, Shi Q, Lu S, Wang X, Zhong Y, Su T, Wang X, Long D, Li Y, Zhang J, Wang L, Long T, Zhu H, Lu X*, Yang H*. Multivalent assembly of nucleolin-targeted F3 peptide potentiates TRAIL's tumor penetration and antitumor effects. J Control Release. 2025;383:113835.
8. Zeng W, Zhang Y, Wang X, Wang S, Lin T, Su T, Jin Y, Yuan Y, Luo M, Zhong Y, Li L, Zhang D, Gong M, Cheng J, Liu J, Liu S*, Wang W*, Yang L*, Yang H*. Chemical Affinity Capture of Plasma Extracellular Vesicles Enables Efficient and Large-Scale Proteomic Identification of Prostate Cancer Biomarkers. ACS Nano. 2025;19(16):15896-15911.
9. Yang H*, Yang G, Lv J, Dai L. Site-Specific Quantitative N-Glycoproteomics: The Great Clinical Application Potential of GlycoQuant. Phenomics. 2024;4(4):394-396.
10. Wang S, Zeng W, Yang Y, Cheng J, Liu D*, Yang H*. DEWNA: dynamic entropy weight network analysis and its application to the DNA-binding proteome in A549 cells with cisplatin-induced damage. Brief Bioinform. 2024;25(6).
11. Wang S, Di Y, Yang Y, Salovska B, Li W, Hu L, Yin J, Shao W, Zhou D, Cheng J, Liu D*, Yang H*, Liu Y*. PTMoreR-enabled cross-species PTM mapping and comparative phosphoproteomics across mammals. Cell Rep Methods. 2024;4(9):100859.
12. Zhang Y, Zeng W, Zhao Y, Yang H*. Editorial: New methods, techniques and applications in clinical glycoproteomics. Front Mol Biosci. 2023;10:1170818.
13. Yang H, Li H, Yang F, Tao Z, Shi Q, She T, Feng Y, Li Z, Chen J, Zhong Y, Su T, Zeng W, Zhang Y, Wang S, Li L, Long T, Long D, Cheng J, Zhu H, Lu X. Molecular superglue-mediated higher-order assembly of TRAIL variants with superior apoptosis induction and antitumor activity. Biomaterials. 2023:121994.
14. Lin T, Chen Z, Luo M, Zhao Y, Zeng W, Zheng S, Su T, Zhong Y, Wang S, Jin Y, Hu L, Zhao W, Li J, Wang X, Wu C, Li D, Liu F, Li G, Yang H*, Zhang Y*. Characterization of site-specific N-glycosylation signatures of isolated uromodulin from human urine. Analyst. 2023;148(20):5041-5049.
15. Zeng W, Zheng S, Su T, Cheng J, Mao Y, Zhong Y, Liu Y, Chen J, Zhao W, Lin T, Liu F, Li G, Yang H*, Zhang Y*. Comparative N-Glycoproteomics Analysis of Clinical Samples Via Different Mass Spectrometry Dissociation Methods. Front Chem. 2022;10:839470.
16. Yu H, Luo H, Chang L, Wang S, Geng X, Kang L, Zhong Y, Cao Y, Wang R, Yang X, Zhu Y, Shi MJ, Hu Y, Liu Z, Yin X, Ran Y, Yang H*, Fan W*, Zhao BQ*. The NEDD8-activating enzyme inhibitor MLN4924 reduces ischemic brain injury in mice. Proc Natl Acad Sci U S A. 2022;119(6).
17. Yang Y, Cheng J, Wang S*, Yang H*. StatsPro: Systematic integration and evaluation of statistical approaches for detecting differential expression in label-free quantitative proteomics. J Proteomics. 2022;250:104386.
18. Wei D, Qi J, Hamblin MR, Wen X, Jiang X*, Yang H*. Near-infrared photoimmunotherapy: design and potential applications for cancer treatment and beyond. Theranostics. 2022;12(16):7108-7131.
19. Zhang Y, Zheng S, Zhao W, Mao Y, Cao W, Zeng W, Liu Y, Hu L, Gong M, Cheng J, Chen Y*, Yang H*. Sequential Analysis of the N/O-Glycosylation of Heavily Glycosylated HIV-1 gp120 Using EThcD-sceHCD-MS/MS. Front Immunol. 2021;12:755568.
20. Zhang Y, Zheng S, Mao Y, Cao W, Zhao L, Wu C, Cheng J, Liu F*, Li G*, Yang H*. Systems analysis of plasma IgG intact N-glycopeptides from patients with chronic kidney diseases via EThcD-sceHCD-MS/MS. Analyst. 2021;146(23):7274-7283.
21. Zhang Y, Zhao W, Mao Y, Chen Y, Zheng S, Cao W, Zhu J, Hu L, Gong M, Cheng J, Yang H*. O-Glycosylation Landscapes of SARS-CoV-2 Spike Proteins. Front Chem. 2021;9:689521.
22. Zhang Y, Zhao W, Mao Y, Chen Y, Wang S, Zhong Y, Su T, Gong M, Du D, Lu X, Cheng J*, Yang H*. Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins. Mol Cell Proteomics. 2021;20:100058. ESI高被引
23. Zeng W, Zheng S, Mao Y, Wang S, Zhong Y, Cao W, Su T, Gong M, Cheng J, Zhang Y*, Yang H*. Elevated N-Glycosylation Contributes to the Cisplatin Resistance of Non-Small Cell Lung Cancer Cells Revealed by Membrane Proteomic and Glycoproteomic Analysis. Front Pharmacol. 2021;12:805499.
24. Chen J#, Yang H#, Feng Y, Shi Q, Li Z, Tao Z, Fan J, Jin Y, Li S, Cheng J, Lu X. A single nucleotide mutation drastically increases the expression of tumor-homing NGR-TNFalpha in the E. coli M15-pQE30 system by improving gene transcription. Appl Microbiol Biotechnol. 2021;105(4):1447-1460.
25. Zhang Y, Zhao W, Zhao Y, Mao Y, Su T, Zhong Y, Wang S, Zhai R, Cheng J, Fang X*, Zhu J*, Yang H*. Comparative Glycoproteomic Profiling of Human Body Fluid between Healthy Controls and Patients with Papillary Thyroid Carcinoma. J Proteome Res. 2020;19(7):2539-2552.
26. Zhang Y, Mao Y, Zhao W, Su T, Zhong Y, Fu L, Zhu J, Cheng J, Yang H*. Glyco-CPLL: An Integrated Method for In-Depth and Comprehensive N-Glycoproteome Profiling of Human Plasma. J Proteome Res. 2020;19(2):655-666.
27. Zhang Y, Lin T, Zhao Y, Mao Y, Tao Y, Huang Y, Wang S, Hu L, Cheng J*, Yang H*. Characterization of N-linked intact glycopeptide signatures of plasma IgGs from patients with prostate carcinoma and benign prostatic hyperplasia for diagnosis pre-stratification. Analyst. 2020;145(15):5353-5362.
28. Wei D, Tao Z, Shi Q, Wang L, Liu L, She T, Yi Q, Wen X, Liu L, Li S, Yang H*, Jiang X*. Selective Photokilling of Colorectal Tumors by Near-Infrared Photoimmunotherapy with a GPA33-Targeted Single-Chain Antibody Variable Fragment Conjugate. Mol Pharm. 2020;17(7):2508-2517.
29. Wang S, Zhu H, Zhou H, Cheng J*, Yang H*. MSpectraAI: a powerful platform for deciphering proteome profiling of multi-tumor mass spectrometry data by using deep neural networks. BMC Bioinformatics. 2020;21(1):439.
30.Wang S, Li W, Hu L, Cheng J, Yang H*, Liu Y*. NAguideR: performing and prioritizing missing value imputations for consistent bottom-up proteomic analyses. Nucleic Acids Res.2020;48(14):e83.
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